Technology

Strain development and fermentation

Probiotics and enzymes play a very important role in different food, feed and pharmaceutical applications since they are robust, amenable for mass production and easier to manipulate. These probiotics and enzymes are manufactured through process of controlled fermentation effectively by optimizing various process parameters. Since fermentation is the most critical part of the production process, we keep developing novel strains, methodologies and optimization strategies to achieve maximum yields from our in-house production processes. We keep screening natural isolates of bacteria for their probiotic potential and develop methods for their mass production. We have developed several microbial strains in our laboratory for the production of enzymes relevant for food, feed and pharmaceutical applications. We produce a range of probiotics and enzymes using both solid state and submerged fermentation technologies and we are always looking into methods and processes that offer better production economics so that we can offer the products to our clients at a competitive price.

Scale up

A scale-up study involves attempts to replicate a laboratory process at successively larger volumes of batches in order to achieve commercial viability and scale. It is very difficult to bring laboratory-level innovations involving enzymes and probiotics into a commercial scale as it involves years of research, multiple iterations and substantial engineering and financial commitment. We have a team of experienced scientists, bioprocess professionals and engineers working in our state-of-the-art facilities to achieve these goals on a regular basis. As a result, we have the capacity to manufacture stable, active and consistent products on commercial scale.

Enzyme Engineering

The advent of in vitro molecular evolution techniques has opened up a new area for enzyme improvement. We combine in vitro gene shuffling and structure-guided recombination to generate maximum variation in primary protein sequences while retaining the original identity of the parent enzyme. Subsequently, we select the superior progeny sequences which have the desired attributes. We are actively working on modifying the enzymes of commercial relevance by combining gene shuffling and structure-guided recombination.

Our engineering toolbox

• Genomic database scanning
• Analysis of structure and sequences
• Random mutagenesis
• Recombination
• Gene shuffling
• CRISPR
• RNAi