Fermentation


Solid-state fermentation (SSF) has been popular method for production of enzymes using agro-industrial residues. Use of agro-industrial waste residues as substrate can avoid the environmental problems caused by its disposal in the environment. Fungi and bacteria can grow on solid substrates and find application in SSF processes. Filamentous fungi are the best adapted for SSF and dominate our SSF research work.

We have developed several strains adapted to SSF in our laboratory for production of multitude of enzymes relevant for food, feed, industrial and environmental applications. At Karyotica we make constant effort to identify high enzyme yielding conditions by optimizing growth conditions and growth medium for superior quality product which is economical to the client.

Fermentation Submerged fermentation (SmF) utilizes free flowing liquid substrates, such as corn steep liquor, molasses and nutrient broths. The enzymes and bioactive compounds are secreted into the fermentation broth. The substrates are utilized quite rapidly and hence need to be constantly supplemented with nutrients. This fermentation technique is best suited for microorganisms such as bacteria that require high moisture.

At Karyotica we have developed cutting edge SmF platform for production various enzymes and many other biocatalysts. All our recombinant range of enzymes are mainly produced through SmF process. We further use SmF for the production of a range of probiotics at our state of the art GMP facility and constantly investigate into methodologies and processes which result in higher yields and better product attributes.

Economically viable production of enzymes, proteins or probiotics requires highly efficient production strains. Karyotica has the technology base in developing such strains for a range of species suited for wide range of industrial enzymes and probiotics.

The advent of in vitro molecular evolution techniques has opened up a new area for enzyme improvement. We combine in vitro gene shuffling and structure guided recombination to generate maximum variation in primary sequences while retaining the original identity of the parent enzyme.

Subsequently we select the superior progeny sequences which have the desired attributes. We are actively working on modifying the most of enzymes of commercial relevance by combining gene shuffling and structure guided recombination

Biocatalysis is the application of enzymes as (bio)catalyst in chemical reactions and has developed into a standard technology. With a suitable enzyme, a reaction can be conducted under mild conditions with high selectivity and efficiency.

The Enzyme route offers many advantages over the chemical route that includes:

  • Reduced reaction time : Increased operating efficiency
  • Specificity : The production of unwanted by-products is avoided and there is no need to extensively refine and purify the desired product
  • Cost Saving : enzymes can be immobilized and therefore re-used several times, providing valuable cost savings
  • Mild conditions: Reaction under mild reaction conditions, low energy costs
  • Environmentally Friendly : enzymes are non corrosive and fully biodegradable
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